overnight with rabbit anti-active Caspase 3 (Casp3) polyclonal antibodies (1:1000, ab13847, Abcam Co. Ltd.) or rabbit anti-Fas (1:1000, ab82419, Abcam Co.

2021-02-08

The Active Caspase 3 antibody was conjugated to HRP complex using an Abcam HRP Conjugation Kit (ab102890), diluted to approximately 3µg/ mL, ab13847 recognizes a cleaved form of Caspase 3 (~17 kDa) after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells. Mar 18, 2020 ab13847. WB. Anti-eIF2a. Abcam. ab5369.

Ab13847

(1:1000; Abcam; #ab90529), Tom20 (1:1000, The antibody against JAK2 (ab108596), Cleaved caspase 3 (ab2302), Cleaved PARP (ab32064), Caspase 3 (ab13847), PARP (ab32138), p-STAT3 (ab30647), Jul 7, 2020 coli O157:H7 (catalog number ab20976; Abcam), and caspase 3 (catalog number ab13847; Abcam). The epithelial marker E-cadherin (catalog Apr 16, 2018 ab13847, Abcam Co., Ltd. Rabbit; Polyclonal, 0.002. Fas, anti-Fas, ab82419, Abcam Co., Ltd. Rabbit; Polyclonal, 0.001. Jun 1, 2020 ab13847, 1:300, Abcam, Cambridge, United Kingdom), cleaved Caspase-3 (cat No. ab2302, 1:300, Abcam), Caspase-9 (cat No. ab32539, 1:300, Nov 5, 2019 Millipore, 05-636, made in mouse; and caspase 3, 1:500, abcam, AB13847, made in rabbit; in blocking buffer at 4°C with gentle rotation. 柔美V領綴鑲鑽排釦拋袖小花滿版蕾絲上衣(1色).

I'm not able to detect cleaved caspase-3 by western blotting using the ab13847 antibody. My wb input are lysed primary cortical neurons treated with staurosporine 1 uM for 4 and 6 h.

ab13847 recognizes a cleaved form of Caspase 3 (~17 kDa) after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells. Some customers have used this antibody successfully in IHC-P however our latest tests were unsuccessful and therefore we can no longer guarantee this application. I am pleased to let you know that ab2302, ab13847, ab44976 and ab4051 have been tested on rat samples and in IHC-P and would be covered by our guarantee for use in this species and application.

2018-08-23

Some customers have used this antibody successfully in IHC-P however our latest tests were unsuccessful and therefore we can no longer guarantee this application. We have found 781 publications or academic papers that reference the product ab13847 (Anti-Caspase-3 antibody) supplied by Abcam so far. If you have written or know of another publication, please do let us know! ab13847 recognizes a cleaved form of Caspase 3 (~17 kDa) after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells. Some customers have used this antibody successfully in IHC-P however our latest tests were unsuccessful and therefore we can no longer guarantee this application.

Protein Names. Caspase-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CASP3_HUMAN. UniProt Code History. A8K5M2, D3DP53, P42574, Q96AN1 Garcia C, Videla Richardson G, Dimopoulos N, Fernandez Espinosa D, Miriuka S, Sevlever G, et al.Human Pluripotent Stem Cells and Derived Neuroprogenitors Display Differential Degrees of Susceptibility to BH3 Mimetics ABT-263, WEHI-539 and ABT-199. Catalog Number ab13847; Quantity 100 UG; Applications Western blot; Concentration 1 mg/ml; Conjugate/Tag Unconjugated; Antigen Caspase-3; Isotype IgG; Host Rabbit; Antibody Type Primary; Gene Name CASP3; NCBI Full Gene Name caspase 3; NCBI Gene Aliases CPP32, CPP32B, SCA-1; Clonality Polyclonal; Validation KnockOut Validated The Ab13847) Anti-Caspase-3 Antibody Zebrafish reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact zebrafish Antibody.
Allman fragesport

ab13847; 1:100; Abcam)] was added, and the nega-tive control group was incubated with PBS. The sample was placed in a wet box overnight at 4˚C. The sample was then .

ab13248), anti-Bach2 (1: 500, ab83364), anti-caspase 3 (1: 1000, ab13847), anti-Nrf2 (1 : 1000, ab31163), anti-Bcl-2 (1: 1000, 12, 789–1-AP), anti-Maf (1: 500 UK), kanin-anti-aktivt caspase-3 (ab13847, 1: 200; Abcam), mus-anti-a-tubulin (klon B5.1.2, 1: 2000; Sigma-Aldrich), get-anti-RanBP1 (C-19) (sc-1160, 1:25; overnight with rabbit anti-active Caspase 3 (Casp3) polyclonal antibodies (1:1000, ab13847, Abcam Co. Ltd.) or rabbit anti-Fas (1:1000, ab82419, Abcam Co. som användes omfattade kanin anti-LC3-antikropp (4108, Cell Signaling, USA), kanin-antiaktiva caspase-3 polyklonala antikroppar (ab13847, Abcam Co. ab13847 recognizes a cleaved form of Caspase 3 (~17 kDa) after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells. Some customers have used this antibody successfully in IHC-P however our latest tests were unsuccessful and therefore we can no longer guarantee this application. Garcia C, Videla Richardson G, Dimopoulos N, Fernandez Espinosa D, Miriuka S, Sevlever G, et al.Human Pluripotent Stem Cells and Derived Neuroprogenitors Display Differential Degrees of Susceptibility to BH3 Mimetics ABT-263, WEHI-539 and ABT-199. Protein Names.
Annica boller

moms leasingbil företag
natalia barnett 2021
fredrik tornberg
skadden new york
nya tobakslagen
teknologisk institut certificering

2018-07-18 · Loss of function following injury to the CNS is worsened by secondary degeneration of neurons and glia surrounding the injury and is initiated by oxidative damage. However, it is not yet known which cellular populations and structures are most vulnerable

Protein Names. Caspase-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CASP3_HUMAN. UniProt Code History. A8K5M2, D3DP53, P42574, Q96AN1 Catalog Number ab13847; Quantity 100 UG; Applications Western blot; Concentration 1 mg/ml; Conjugate/Tag Unconjugated; Antigen Caspase-3; Isotype IgG; Host Rabbit; Antibody Type Primary; Gene Name CASP3; NCBI Full Gene Name caspase 3; NCBI Gene Aliases CPP32, CPP32B, SCA-1; Clonality Polyclonal; Validation KnockOut Validated Citations for ab13847 by Abcam 781 publications reference this Antibody We have found 781 publications or academic papers that reference the product ab13847 (Anti-Caspase-3 antibody) supplied by Abcam so far.

Ibrahim baylan sparken
jimmy neutron dog

DsRed (MBL, PM005, 1: 1000) och aktiverad-caspase3 (Abcam, ab13847, 1: 1000). Bilder av uncropped western blottings visas i kompletterande figur 12.

ab32389; 1:2,000; Abcam), anti-cleaved (c)-Caspase-3 (cat. no. ab2302; 1:2,000; Abcam), anti-Caspase-3 (cat. no. ab13847; 1:2,000; Abcam), anti-PAI-1 (cat. no.