IPTG (Isopropyl-β-D-thiogalactopyranoside) is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.

IPTG is a non-metabolizable galactose analog that induces expression of the lac operon in Escherichia coli.2,3 IPTG functions by binding to the lac repressor.

Freeze at -20 °C. Induction of protein expression is usiually accomplished with an IPTG concentration of 0.5-1 mM. IPTG Dissolve 1.2g IPTG (isopropyl-β-d-thiogalactopyranoside) in deionized water to a final volume of 50ml. Filter sterilize (0.2µm), and store in 5ml aliquots at –20°C. The final concentration of IPTG is 0.1M. The stock solution of IPTG is stable at this temperature for 2–4 months. IPTG is commonly used with X-Gal, Bluo-Gal and Magenta-Gal in molecular biology labs because of its ability to induce the lac operon without being metabolize Isopropyl β-d-1-thiogalactopyranoside (IPTG) is a molecular biology reagent.

Iptg stock

IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. 1 M IPTG Stock Solution (isopropyl Beta-D-thioglucopyranoside, MW 238.3 g/mol) 2.383 g IPTG in 10 mL ddH2O. Filter sterilize with 0.22 um membrane cartridge. or buy 25 mL 1M stock solution Teknova 13431 (Fisher) Store at -20C You can make 1M concentarion stock of IPTG and add the volume accordingly as you want and as per your culture volume. Cite.

UPDATE: Some items may be delayed in shipping or out of stock due to extremely high demand. Corning® 1 g IPTG (Isopropylthiogalactoside), Powder.

IPTG is commonly used with X-Gal, Bluo-Gal and Magenta-Gal in molecular biology labs because of its ability to induce the lac operon without being metabolize Isopropyl β-d-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator Se hela listan på sigmaaldrich.com Details.

IPTG is widely used to induce the expression of cloned genes under the control of the lac operon and to screen blue/white colony in X-gal plate. Note: To prepare 10 mL 1M IPTG stock solution, weight 2.38 g IPTG and dissolve in 8 ml distilled water, adjust to a final volume of 10 ml with distilled water.

IPTG (Isopropyl ß-D-1-thiogalactopyranoside), is a molecular biology reagent.

IPTG (Isopropyl Thio-beta-D-Galactoside, MW 238.31, Fisher BP1755-1) 1 M. 0.2383 g.
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Dissolve 4.2 g IPTG in 100 ml deionized water. •. After total dissolution, sterile filtration using 0.2 µm filter.

Preparation Instructions IPTG is soluble at 250 mg/ml in water and may be IPTG Induction Theory Isopropyl β-D-1-thiogalactopyranoside (IPTG, also known as lad-y) is a molecular biology reagent.
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IPTG Stock Quote. Volume Open Day's Low Day's High 52 Wk Low 52 Wk High Bid Ask EPS PE Ratio Shares Market Cap Dividend Ex-Div Date Yield. 1 day 5 days 10 days 1

3. Add 40 μL of the Thermo Scientific X-Gal Solution (20 mg/mL), ready-to-use (Cat #R0941). Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose.

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Usually works just fine and protein production is great.